Purification and characterization of two fructose diphosphate aldolases from Escherichia coli (Crookes' strain)
نویسندگان
چکیده
منابع مشابه
Purification and characterization of two fructose diphosphate aldolases from Escherichia coli (Crookes' strain).
Two fructose diphosphate aldolases (EC 4.1.2.13) were detected in extracts of Escherichia coli (Crookes' strain) grown on pyruvate or lactate. The two enzymes can be resolved by chromatography on DEAE-cellulose at pH7.5, or by gel filtration on Sephadex G-200, and both have been obtained in a pure state. One is a typical bacterial aldolase (class II) in that it is strongly inhibited by metal-ch...
متن کاملPurification and characterization of P fimbriae from an Escherichia coli strain isolated from a septicemic turkey.
A pap+ Escherichia coli isolate from a turkey with colisepticemia expressed P fimbriae with a major subunit of an apparent molecular mass of 18 kDa which reacted with anti-F11 serum. This fimbriae was purified and polyclonal antiserum was produced in rabbits. The N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of F11. On immunoblotting,...
متن کاملPhysicochemical characterization of ribonucleoside diphosphate reductase from Escherichia coli.
Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, proteins Bl and B2. Affinity chromatography resulted in a Bl preparation which appeared homogeneous during polyacrylamide gel electrophoresis and ultracentrifugation. The molecular weight was 160,000 by sedimentation equilibrium centrifugation using a partial specific volume of 0.706 ml per g at 4”. Protein Bl...
متن کاملProperties of fructose 1,6-diphosphate aldolases from spores and vegetative cells of Bacillus cereus.
Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties. Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobili...
متن کاملProperties of Fructose 1,6-Diphosphate Aldolases from Spores and Vegetative Cells of Bacillus cereus1
Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties: Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobili...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1973
ISSN: 0264-6021
DOI: 10.1042/bj1310833